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目的测定不同采收期珠子参药材中竹节参皂苷Ⅳa的含量并确定珠子参的最佳采收期。方法供试品用60%乙醇超声提取40 min,滤过,取续滤液作为供试品溶液,高效液相色谱仪测定峰面积。色谱柱:Kromasil-C18(5 um,4.6 mm×150 mm);流动相:乙腈-0.2%磷酸溶液(36:64);柱温:30℃;流速:1.0 mL/min;检测波长:203 nm。结果不同采收期珠子参中竹节参皂苷Ⅳa的累积量差异较大且7月含量最高。结论以竹节参皂苷Ⅳa为指标性成分确定珠子参的最佳采收期应为7月份。
Objective To determine the content of bamboo ginseng saponin Ⅳa in different harvesting time and determine the optimal harvest time of the beads. Methods The samples were sonicated with 60% ethanol for 40 min, filtered, and the filtrate was taken for the test solution, the peak area was determined by high performance liquid chromatography. Column: Kromasil-C18 (5 um, 4.6 mm × 150 mm); mobile phase: acetonitrile- 0.2% phosphoric acid solution (36:64); column temperature: 30 ° C .; flow rate: 1.0 mL / min; . Results The differences of accumulation amount of bamboo ginsenoside Ⅳa in different harvest time were significant and the content in July was the highest. Conclusion The best harvesting time for determination of Begonia ginseng with indications of bamboo ginsenoside Ⅳa should be July.