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目的探讨拟除虫菊酯对大鼠脑组织中谷氨酸和天门冬氨酸含量的影响及其可能机理。方法用反相高效液相色谱技术观察溴氰菊酯和氯菊酯处理后大鼠脑组织皮层、海马、小脑中谷氨酸和天门冬氨酸含量的变化，并应用电压依赖性钙通道阻断剂尼莫地平和一氧化氮合酶竞争性抑制剂Ｌ硝基精氨酸探讨Ｃａ２＋和一氧化氮对溴氰菊酯和氯菊酯作用的影响。结果溴氰菊酯和氯菊酯可以明显提高大鼠脑组织皮层、海马、小脑中谷氨酸和天门冬氨酸的含量；尼莫地平能有效地阻断溴氰菊酯和氯菊酯升高谷氨酸和天门冬氨酸的作用；Ｌ硝基精氨酸也能完全阻断溴氰菊酯的作用，但只能部分阻断氯菊酯的作用。结论拟除虫菊酯能够提高大鼠脑组织中谷氨酸和天门冬氨酸的含量；Ｃａ２＋和一氧化氮在溴氰菊酯和氯菊酯升高谷氨酸和天门冬氨酸的机制中起着十分重要的作用；溴氰菊酯和氯菊酯升高谷氨酸和天门冬氨酸的机理可能存在着差异
Objective To investigate the effect of pyrethroid on glutamate and aspartate content in rat brain and its possible mechanism. Methods The contents of glutamic acid and aspartic acid in cortex, hippocampus and cerebellum of rat treated with deltamethrin and permethrin were determined by reversed-phase high-performance liquid chromatography (HPLC). The voltage-dependent calcium channels were used to block the changes Nimodipine and nitric oxide synthase competitive inhibitor L-nitroarginine to investigate the effects of Ca2 + and nitric oxide on deltamethrin and permethrin. Results Deltamethrin and permethrin significantly increased the content of glutamate and aspartate in the cortex, hippocampus and cerebellum of rat brain. Nimodipine could effectively block the increase of deltamethrin and permethrin L-arginine and aspartate; L-nitroarginine also completely blocked the role of deltamethrin, but only partially blocked the role of permethrin. Conclusion Pyrethroids can increase glutamate and aspartate levels in rat brain tissue. Ca2 + and nitric oxide play a very important role in the mechanism of deltamethrin and permethrin increasing glutamate and aspartate Important role; deltamethrin and permethrin may increase the mechanism of glutamate and aspartate differences may exist