论文部分内容阅读
目的 研究 5 0Hz工频电磁场对体外培养的小鼠骨髓间充质干细胞的增殖、分化及细胞内环磷酸腺苷 (cAMP)水平的影响。方法 体外培养小鼠骨髓间充质干细胞 ,取第 3代细胞 ,应用工频电磁场及成骨性诱导剂干预 ,并分别于第 3天和第 5天用MTT法检测其增殖活性 ,用放免法检测细胞内cAMP浓度 ,于第 7天检测细胞内碱性磷酸酶 (ALP)的活性。结果 频率 5 0Hz、强度 2mT的正弦波电磁场能抑制体外培养的小鼠骨髓间充质干细胞的增殖 (P <0 .0 5 ) ,使其ALP活性增高 (P <0 .0 5 ) ,在磁场作用的早期 ( 1～ 3d) ,细胞内的cAMP水平明显增高 (P <0 .0 1) ,继续暴磁至第 5天 ,cAMP水平不再发生变化 (P >0 .0 5 )。结论 5 0Hz工频电磁场可能通过作用于cAMP 蛋白激酶A信号转导系统 ,从而调控体外培养的小鼠骨髓间充质干细胞的增殖与分化 ,此调控作用可能发生在细胞受电磁场作用的早期。
Objective To investigate the effects of 50 Hz electromagnetic fields on the proliferation, differentiation and cAMP of mouse bone marrow mesenchymal stem cells cultured in vitro. Methods Mice bone marrow mesenchymal stem cells were cultured in vitro. The third generation cells were harvested and the cells were exposed to commercial electromagnetic fields and osteogenic inducers. The proliferative activity was detected by MTT assay on day 3 and day 5 respectively. Intracellular cAMP concentrations were measured and intracellular alkaline phosphatase (ALP) activity was measured on day 7. Results Sine wave electromagnetic field with frequency of 50Hz and intensity of 2mT could inhibit the proliferation of mouse bone marrow mesenchymal stem cells cultured in vitro (P <0.05) and increase the ALP activity (P <0.05) In the early stage of action (1 ~ 3d), the level of intracellular cAMP was significantly increased (P <0.01). The levels of cAMP did not change after the first 5 days (P> 0.05). Conclusion 50 Hz power frequency electromagnetic field may regulate the proliferation and differentiation of mouse bone marrow mesenchymal stem cells cultured in vitro by acting on the cAMP protein kinase A signal transduction system. This regulation may take place in the early stage of the cell by the electromagnetic field.