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目的研究人重组粒细胞集落刺激因子(rhG-CSF)动员的健康供者外周血采集物(G-PB)和稳态骨髓采集物(SS-BM)在免疫学特性方面的异同。方法对2003年4月至10月北京大学血液病研究所接受异基因造血干细胞移植的15名亲缘供者,采用流式细胞仪测定两种移植物的T细胞亚群、树突细胞(DC)亚群、单核细胞及共刺激分子CD28的表达,并用四甲基偶氮唑盐(MTT)法和酶联免疫吸附法(ELISA)检测G-PB和SS-BM中T淋巴细胞增殖能力和白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)的分泌情况。结果G-PB中淋巴细胞,CD3+、CD3+CD4+、CD3+CD8+T细胞,单核细胞的含量以及CD4与CD8比值均高于SS-BM(P<0.01);G-PB的T细胞增殖能力与SS-BM相比差异无显著性意义(P>0.05);每微升G-PB移植物中T细胞分泌细胞因子IFN-γ、IL-4的量均高于SS-BM(P<0.05、P<0.01),G-PB中IL-4与IFN-γ比值与SS-BM比较差异无显著性意义;G-PB中DC1、DC2的含量高于SS-BM(P<0.01),DC2与T淋巴细胞的比值低于SS-BM(P<0.01),单核细胞和T淋巴细胞的比值显著高于SS-BM(P<0.01);CD3+、CD4+、CD8+细胞上CD28表达的百分比和总体表达均高于SS-BM(P<0.01);CD8+CD28-T细胞与T淋巴细胞的比值和SS-BM的差异无显著性意义(P>0.05)。结论G-PB与SS-BM在免疫特性方面的异同可能是二者在移植后具有相似的急性移植物抗宿主病(GVHD)发生率以及G-PB移植后较高的慢性GVHD发生率和较低复发率的免疫学基础。
Objective To investigate the immunological characteristics of G-PB and SS-BM collected from patients with rhG-CSF mobilized. Methods Totally 15 neonates with allogeneic hematopoietic stem cell transplantation were enrolled in the Institute of Hematology, Peking University from April to October 2003. The T lymphocyte subsets, dendritic cells (DCs) Subunit, monocyte and costimulatory molecule CD28 were detected. The proliferation of T lymphocytes in G-PB and SS-BM were detected by MTT assay and enzyme-linked immunosorbent assay (ELISA) Interleukin-4 (IL-4), interferon-γ (IFN-γ) secretion. Results The levels of lymphocytes, CD3 +, CD3 + CD4 +, CD3 + CD8 + T cells and monocytes in G-PB and the ratio of CD4 to CD8 were higher than that in SS-BM (P <0.01) (P> 0.05). The amount of cytokines IFN-γ and IL-4 secreted by T cells per microliter of G-PB grafts were higher than that of SS-BM (P < 0.05, P <0.01). The ratio of IL-4 to IFN-γ in G-PB had no significant difference compared with SS-BM. The contents of DC1 and DC2 in G-PB were higher than those in SS- The ratio of DC2 to T lymphocytes was lower than that of SS-BM (P <0.01), and the ratio of monocytes to T lymphocytes was significantly higher than that of SS-BM (P <0.01). The percentage of CD28 on CD3 +, CD4 + and CD8 + (P <0.01). The ratio of CD8 + CD28-T cells to T lymphocytes and SS-BM had no significant difference (P> 0.05). Conclusions The similarities and differences in immunological characteristics between G-PB and SS-BM may be related to similar incidence of GVHD after transplantation and higher incidence of chronic GVHD after G-PB transplantation Low recurrence rate of immunological basis.