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帕金森氏病治疗药左旋多巴与芳香族氨基酸脱羧酶抑制剂如卡比多巴等共用所产生的运动机能波动是治疗中存在的主要问题.为研究其原因需检测此类药物与其代谢产物的浓度.本文报道用电化学检测HPLC法,单次采集1ml血浆可同时测定左旋多巴及其代谢物3-O-甲基多巴、多巴胺和卡比多巴的含量,方法可靠、快速、准确.作者采用的HPLC仪器包括Varian8500泵,5μm颗粒Partisil-5 ODS-3色谱柱(100×4.6mm,id)电化学检测器及Vista CDS401积分器.电化学检测器的电流检测系统包括LCC231薄层流通电化学小池E230玻璃碳工作电极和甘汞参比电极,检测器电位为
Parkinson’s disease treatment drugs L-DOPA and aromatic amino acid decarboxylase inhibitor such as carbidopa sharing exercise motor function fluctuations is the main problem in the treatment of the reasons for the need to detect such drugs and their metabolites The concentration of 3-O-methyldopa, dopamine and carbidopa in levodopa and its metabolites can be simultaneously determined by single-step collection of 1 ml plasma.The method is reliable, rapid, The HPLC instrument used by the author includes a Varian 8500 pump, a 5 μm particle Partisil-5 ODS-3 column (100 × 4.6 mm, id) electrochemical detector and a Vista CDS401 integrator. The electrochemical detector’s current sensing system includes the LCC231 Layer flow through the electrochemical cell E230 glassy carbon working electrode and calomel reference electrode, the detector potential