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目的采用标准近交系小鼠C57BL建立麻痹性贝类毒素(PSP)检测方法,为贝类食品中该毒素的检测提供可使用的实验动物品系资料。方法对近5年广州及深圳发生疑似PSP中毒的样品同时采用C57BL小鼠单位法和免疫学试剂盒法进行检测,分析二者结果的一致性。结果C57BL小鼠单位法与免疫学试剂盒检测法这一欧盟的标准的检测方法相比,结果具有明显的关联性,关联系数r=0.86。结论C57BL小鼠单位法可用于确定贝类食品是否污染PSP的检测。
Objective To establish a paralytic shellfish poison (PSP) assay using standard inbred mouse C57BL and to provide experimental animal strains data for the detection of the toxin in shellfish food. Methods Samples of suspected PSP poisoning in Guangzhou and Shenzhen in the recent 5 years were tested both by C57BL mouse unit method and immunological kit method, and the consistency of the results was analyzed. Results The C57BL mouse unit method was significantly correlated with the standard test method of immunological test kit, which is the EU standard. The correlation coefficient was 0.86. Conclusion The C57BL mouse unit method can be used to determine whether shellfish contaminated PSP detection.