基于VP1重组蛋白的口蹄疫病毒O型间接ELISA检测技术

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采用RT-PCR方法扩增O型口蹄疫病毒VP1基因片段,将其克隆于pET-32a.将重组质粒转化宿主菌Rosetta,经1.0 mmol/L IPTG诱导,外源基因获高效表达.通过Western-blot以及ELISA试验证明,重组VP1蛋白与标准阳性血清发生反应.以纯化的VP1蛋白作为检测抗原包被酶标板建立了检测口蹄疫病毒O型抗体的间接ELISA方法.对355份临床血清样品的检测结果显示,建立的方法与口蹄疫病毒O型液相阻断试剂盒符合率为98.87%.表明建立的方法能够用于口蹄疫病毒O型抗体的快速分型检测.
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