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目的:加速成骨正畸能增加骨改建及牙移动的速率,但仅仅只有部分关于加速成骨正畸的机制被获知。本实验进一步探讨骨改建过程中破骨细胞的分化形成情况,以更好地了解加速成骨正畸的机制。方法:40只新西兰大白兔随机分成5组,每组8只。下颌左侧作为加速成骨正畸实验侧,右边作为常规正畸对照侧。左右两侧均用4盎司的力值。在1、3、5、7、14d将实验动物处死。每个时间点上的3只动物用作形态学观察,另外5只用作分子生物研究。结果:形态学观察显示,加速成骨实验组压力侧破骨细胞计数及骨改建活跃程度均较对照组高,而且有2次连续明显的增长。在加速成骨正畸(AOO)中,可观察到破骨细胞不同的分化因子在同一时期表达趋势不同:在第5天,实验组,CTSK,TRAP的mRNA显著上调并达到高峰;CTR的mRNA表达量在1~7d较平缓;JDP2、NFATC1的mRNA的表达量趋势相一致,在第7天达到高峰;Fra2的mRNA在第5天开始增加,之后持续升高。结论:提示在AOO过程中,破骨细胞可能有2次明显的连续分化,继而能持续的加速牙齿移动。
OBJECTIVE: To accelerate osteogenesis orthodontics can increase the rate of bone remodeling and tooth movement, but only some of the mechanisms for accelerating osteogenesis and orthodontics are known. This experiment further explored the formation of osteoclast differentiation during bone remodeling in order to better understand the mechanism of accelerating osteogenesis and orthodontics. Methods: Forty New Zealand white rabbits were randomly divided into five groups of eight. The left side of mandibular as an experimental side to accelerate osteogenesis and orthodontics, the right side as a routine orthodontic control side. Use 4 ounces of force on each side. The experimental animals were sacrificed on days 1,3,5,7 and 14. Three animals at each time point were used for morphological observation, and the other five were used for molecular biology research. Results: Morphological observation showed that the number of osteoclast on the pressure side and the degree of bone remodeling in accelerated osteogenesis group were higher than those in control group, and there were two consecutive and significant increases. In accelerated osteogenesis and orthodontics (AOO), different differentiation factors of osteoclasts could be observed in the same period: the mRNA and protein levels of CTR and TRAP in experimental group, CTSK and TRAP were significantly up-regulated The mRNA expression of JDP2 and NFATC1 tended to be the same on the 7th day. The mRNA of Fra2 began to increase on the 5th day and then continued to increase. Conclusion: It is suggested that during AOO, osteoclasts may have two distinct and continuous differentiations, and then continue to accelerate tooth movement.