论文部分内容阅读
目的:通过急性毒性实验和蓄积毒性实验对藤黄醇提取物进行安全性评价。方法:急性毒性实验采用灌胃1次的给药方式,观察给药期间小鼠的一般情况并采用SPSS软件计算其半数致死量;蓄积毒性实验采用蓄积系数法中递增剂量法进行实验,观察给药期间小鼠的一般情况、检测脏器系数和血液生化指标并对重要脏器进行病理切片。结果:藤黄醇提取物的半数致死量LD50为1.691 g/kg(95%CI:1.149~5.030 g/kg)。藤黄醇提取物的蓄积系数为2.92,有明显蓄积毒性,对肝、肺、脑、脾、肾均有不同程度的影响,第15天累积灌胃藤黄醇提取物剂量达到4.94 g/kg时,蓄积毒性实验中实验组昆明小鼠死亡过半。结论:藤黄醇提取物的急性毒性级别为低毒性,其蓄积毒性表现主要是肝毒性。
OBJECTIVE: To evaluate the safety of the extract of pentacontide by acute toxicity test and accumulative toxicity test. Methods: The acute toxicity test was given by intragastric administration once. The general situation of the mice during the administration was observed and the median lethal dose (LD50) was calculated by SPSS software. The accumulative toxicity experiment was conducted by increasing dose method with the accumulation coefficient method, The general situation of mice during the test, detection of organ coefficients and blood biochemical indicators and pathological sections of important organs. Results: The LD50 of decyl alcohol extract was 1.691 g / kg (95% CI: 1.149 ~ 5.030 g / kg). The accumulation coefficient of pentylenetetrahydrofolate extract was 2.92, which showed obvious accumulative toxicity and had different effects on the liver, lung, brain, spleen and kidney. The cumulative intragastric administration of pentylenetetrahydrofuran extract reached 4.94 g / kg on the 15th day When accumulating toxicity experiment, experimental group Kunming mice died more than half. CONCLUSION: The acute toxic level of pentylenetetramine extract is low toxicity, and its accumulative toxicity is mainly hepatotoxicity.