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目的探讨R,S和R/S型3-氯-1,2-丙二醇(3-MCPD)对ICR小鼠的急性毒性。方法选用健康性成熟ICR小鼠,对R,S及R/S型3-MCPD染毒剂量依次为176.78、198.43、222.73、250、280.61、314.98和353.55、396.84、445.44和499.99mg/kg,89.09,100,112.25,125.99,141.42,158.74和178.18mg/kg及130.25、150、172.75、198.95、229.13、263.88和303.91mg/kg。经口一次灌胃染毒观察14天,以改良寇式法计算LD50,计算脏体比并进行肝肾病理学检查。结果R,S及R/S型3-MCPD的LD50及95%可信区间(95%CI)分别为290.54mg/kg(280.74~300.68),117.57mg/kg(113.82,121.45),190.73mg/kg(177.76~204.59)。R型异构体在250mg/kg及以上剂量组动物肾体比显著增加(P<0.05),脑体比在353.55mg/kg、445.44mg/kg及最高剂量组亦显著增大(P<0.05);S型3-MCPD染毒组动物各脏体比与对照组比均无显著差异;R/S型3-MCPD,在198.95mg/kg及以上剂量组动物肾体比明显增加,在229.13mg/kg及以上剂量动物脑体比亦明显增加,与对照组比有差异显著(P<0.05)。在353.55mg/kg及以上剂量R型异构体引起肝细胞肿胀、肝窦扩张和明显充血,在229.13mg/kg及以上剂量(R/S)型3-MCPD也引起肝细胞肿胀和肝窦充血,S型则未引起。R、S及(R,S)型均未引起肾脏明显的病理改变。结论三种3-MCPD急性毒性大小依次为S型>R/S型>R型,R、S型异构体均显示出神经毒性。
Objective To investigate the acute toxicity of R, S and R / S 3-chloro-1,2-propanediol (3-MCPD) to ICR mice. Methods Healthy, mature ICR mice were selected and the doses of 3-MCPD for R, S and R / S were 176.78, 198.43, 222.73, 250, 280.61, 314.98 and 353.55, 396.84, 445.44 and 499.99 mg / kg, respectively, 89.09 , 100, 112.25, 125.99, 141.42, 158.74 and 178.18 mg / kg and 130.25, 150, 172.75, 198.95, 229.13, 263.88 and 303.91 mg / kg. Oral gavage once a day for 14 days, the LD50 was calculated by improved koala method, and the ratio of dirty body to the liver and kidney was calculated. Results The LD50 and 95% CI of R, S and R / S 3-MCPD were 290.54 mg / kg (280.74-300.68), 117.57 mg / kg (113.82,121.45) and 190.73 mg / kg (177.76 ~ 204.59). R ratio increased significantly (P <0.05) in the group of 250mg / kg and above, and the body weight increased significantly in the group of 353.55mg / kg, 445.44mg / kg and the highest dose (P <0.05 ). Compared with the control group, there was no significant difference in the ratio of each dirty body in the S-3-MCPD group compared with the control group. The R / S type 3-MCPD increased significantly in the group of 198.95mg / kg and above, Compared with the control group, the ratio of brain to body in mg / kg and above animals also increased significantly (P <0.05). The R type isomer at 353.55 mg / kg and above caused hepatocyte swelling, sinusoidal dilatation and marked hyperemia. 3-MCPD at a dose of 229.13 mg / kg and above (R / S) also caused hepatocyte swelling and sinusoidal Congestive, S-type is not caused. R, S and (R, S) type did not cause significant renal pathological changes. Conclusion The acute toxicity of three kinds of 3-MCPD is S type> R / S type> R type, R, S type isomers show neurotoxicity.