论文部分内容阅读
目的观察补肾活血方对去卵巢大鼠子宫的雌激素受体及周期蛋白(Cyclin)B表达的影响,探讨其治疗围绝经期综合征的作用机制。方法选择SD雌性大鼠54只,随机分为假手术组、模型组、雌二醇组、补肾活血方组、联合用药组、补肾活血方+假手术组,每组9只。以双侧卵巢切除法建立大鼠围绝经期模型。各组灌胃给药40 d后取血清和子宫称重;酶联免疫吸附(ELISA)法检测血清中雌二醇(E2)、黄体生成素(LH)、卵泡刺激素(FSH)浓度;HE染色观察子宫组织病理改变并测定子宫内膜厚度;免疫组化法观察雌激素受体(ER)α、ERβ在子宫组织中分布与表达情况;蛋白质印迹法(Western blot)法检测子宫组织中周期蛋白(Cyclin B)蛋白表达。结果补肾活血方治疗40 d后,与模型组比较,血清LH、FSH明显降低(P<0.05或P<0.01),血清E2水平升高(P<0.05);同时,子宫脏器指数、子宫内膜厚度、子宫组织中ERα、ERβ和Cyclin B蛋白表达均明显升高(P<0.05),与雌激素组呈现相似的作用,但作用强度均低于雌激素;补肾活血方+假手术组大鼠各项指标与假手术组比较无明显差异。结论补肾活血方具有雌激素样作用,但作用强度弱于雌激素,对正常大鼠子宫组织无显著影响。
Objective To observe the effects of Bushen Huoxue Recipe on the expression of estrogen receptor and Cyclin B in the uterus of ovariectomized rats and its mechanism of action in treating perimenopausal syndrome. Methods Fifty-four female SD rats were randomly divided into sham-operation group, model group, estradiol group, Bushen Huoxue Decoction group, combination therapy group and Bushen Huoxue Recipe + sham operation group, with 9 rats in each group. Perimenopausal model was established by bilateral ovariectomy. Serum and uterus were weighed after gavage administration for 40 days. Serum estradiol (E2), luteinizing hormone (LH) and follicle stimulating hormone (FSH) concentrations were measured by enzyme-linked immunosorbent assay (ELISA) The histopathological changes of uterus were observed by staining and the thickness of endometrium was determined. The distribution and expression of estrogen receptor (ER) α and ERβ in uterine tissue were observed by immunohistochemistry. The expression of ERβ in uterine tissue was detected by Western blotting. Protein (Cyclin B) protein expression. Results After Bushenhuoxue Decoction was given for 40 days, the levels of LH and FSH in serum were significantly decreased (P <0.05 or P <0.01) and the level of serum E2 was increased in the model group (P <0.05) Membrane thickness and the expression of ERα, ERβ and Cyclin B in uterine tissue were significantly increased (P <0.05), but similar to those in estrogen group, but the effect intensity was lower than that of estrogen; Bushen Huoxue Fang + sham operation group There was no significant difference between rats and sham operation group. Conclusion Bushen Huoxue Fang has estrogen-like effect, but its effect intensity is weaker than estrogen, and has no significant effect on uterus tissue of normal rats.