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目的 :探讨受体外周血IgG、MΦ、NK、CD4、CD8及MLR的变化与异种移植物存活的关系。方法 :选用小鼠移植于大鼠耳后心肌组织模型 ,按实验所设Ⅰ、Ⅱ、Ⅲ、Ⅳ组 :在术前 12、8、4、0d及 10、6、2、0d分别将小鼠脾细胞 1× 10 8个及抗脾细胞血清 0 2ml静注大鼠 ,术日始CsA 10mg (kg·d) ,Cy 2 0mg (kg·d) ,CD4McAb、CD8McAb、MΦMcAb、NKmAb均为 2 5 0 μg (kg·d) ,CCV 0 2mg (kg·d)腹腔注射至术后 6d。结果 :Ⅱ、Ⅲ、Ⅳ组移植小鼠心肌存活延长 ,尤以Ⅳ组存活时间达 2 1d左右 (P <0 0 5 ) ,术后 7d外周血MΦ、NK细胞显著增多 (P <0 0 1) ,并维持至术后 2 1d ;移植术后 7dCD4、CD8稍增多 ,术后 2 1d显著增加 (P <0 0 5 ) ;术后 7dⅡ、Ⅲ、Ⅳ组IgG显著下降 (P <0 0 5 ) ;术后 7,2 1d动态MLR比较无显著差异 (P >0 0 5 )。结论 :外周血IgG、MΦ、NK细胞的变化可作为监测异种移植细胞性排斥反应的指标 ,异种移植物存活时间延长 ,将面对宿主T细胞免疫应答。
Objective: To investigate the relationship between the changes of peripheral blood IgG, MΦ, NK, CD4, CD8 and MLR and the survival of xenografts. Methods: The mice were transplanted into the auricular myocardium model of the ear. According to the experimental groups Ⅰ, Ⅱ, Ⅲ and Ⅳ, mice were respectively sacrificed at 12, 8, 4, 0 and 10, 6, 1 × 10 8 spleen cells and 0 ml anti-splenic serum were intravenously injected into the rats. The daily dose of CsA 10 mg (kg · d), Cy 2 0mg (kg · d), CD4McAb, CD8McAb, MΦMcAb and NKmAb were 25 0 μg (kg · d), CCV 0 2mg (kg · d) intraperitoneal injection to 6d after surgery. Results: The survival of myocardium in group Ⅱ, Ⅲ and Ⅳ was prolonged, especially in group Ⅳ (P <0.05), and the number of MΦ and NK cells in 7 days after operation was significantly increased (P <0.01 ), And maintained until 21d after operation. On the 7th day after transplantation, the numbers of CD4 and CD8 increased slightly, and increased significantly 21 days after operation (P <0.05). IgG in group II, III and IV decreased significantly on the 7th day after operation (P <0.05 ). There was no significant difference in dynamic MLR between 7 and 21 days after operation (P> 0.05). CONCLUSION: The changes of peripheral blood IgG, MΦ and NK cells can be used as indicators to monitor the rejection of xenograft cells. The survival time of xenografts is prolonged and will face the immune response of host T cells.