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应用聚合酶链反应检测51例慢性肝炎,19例慢性HBsAg携带者(ASC)和26例HBV感染后的健康者血清中的HBV DNA。结果14例HBeAg阳性患者HBV DNA全部阳性,37例HBeAg阴性者中24例阳性(64.9％)。其中HBV·M阴性9例,有6例阳性。5例抗-HBs阳性者3例阳性。ASC和健康者PCR-HBV DNA检出率分别为36.8％和26.9％,明显低于慢性肝炎的74.5％。结果提示,慢性肝炎患者HBeAg阴性甚至HBV·M阴性时,往往大部分仍有HBV复制,且可能与病变活动有关。ASC和健康者总体病毒复制水平较低,可能与无明显的肝脏病表现有关。
Polymerase chain reaction was used to detect HBV DNA in sera from 51 chronic hepatitis patients, 19 chronic HBsAg carriers (ASC) and 26 healthy controls. Results All 14 HBeAg positive patients had positive HBV DNA, 24 of 37 HBeAg negative patients (64.9%). Of which HBV · M negative in 9 cases, 6 cases were positive. 5 cases of anti-HBs positive in 3 cases were positive. The detection rates of PCR-HBV DNA in ASC and healthy subjects were 36.8% and 26.9% respectively, significantly lower than 74.5% of those with chronic hepatitis. The results suggest that chronic hepatitis patients HBeAg negative even HBV · M negative, the majority of HBV replication is still often, and may be related to the activity of the disease. The overall level of virus replication in ASC and healthy subjects is low, which may be related to the absence of obvious liver disease.