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Objective: To study the protective action of Astragalus Injection (AI) on ouabain-induced cardiac toxicity. Methods: Forty guinea-pigs were randomly divided into the AI group and the control group, AI injected intravenously in the AI group and 0.9% normal saline injected in the control group, ouabain was injected in contralateral of both groups intravenously 8 mins later. The time of cardiac ventricular tachycardia (VT) and cardiac ventricular fibrillation (VF), and the dose of ouabain were documented.Results: Compared with the control group AI could markedly prolong the time of VT and VF, and increase the dose of ouabain induces VT and VF (P<0.01). Conclusion: AI decreases the incidence of digitalism.