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目的检测人创伤大脑皮层与正常大脑皮层基因表达谱的差异,筛选差异表达基因,寻找脑创伤基因治疗的新靶点。方法利用含21 329个基因的人全基因组寡核苷酸芯片,检测5例创伤大脑皮层组织与1例正常对照的差异表达基因。并随机挑选2个差异表达基因做定量聚合酶链反应(PCR)验证。结果5例创伤脑组织共同差异表达的基因数为87个,其中上调的60个,有16个功能未知或为表达序列标签(ESTs)片段,44个分属于12类功能;下调基因27个,有20个功能未知或为EST片段,7个分属于4类功能。定量PCR验证结果提示芯片结果可信。结论基因芯片筛选出的人创伤大脑皮层的差异表达基因大部分在以前的颅脑创伤文献中未曾报道,对其干预有望成为颅脑创伤基因治疗的新靶向。
Objective To detect the difference of gene expression profiles between traumatic cortex and normal cerebral cortex and to screen differentially expressed genes for new targets of gene therapy for traumatic brain injury. Methods Human genome-wide oligonucleotide microarrays containing 21 329 genes were used to detect differentially expressed genes in 5 cortical tissues of traumatic brain and 1 normal control. Randomly selected two differentially expressed genes and verified by quantitative polymerase chain reaction (PCR). Results The number of genes differentially expressed in 5 cases of traumatic brain tissue was 87, of which 60 were up-regulated, 16 of them were unknown or expressed as ESTs, 44 belonged to 12 functions, 27 genes were down-regulated, There are 20 functions unknown or EST fragments, 7 points belong to 4 functions. Quantitative PCR validation results suggest that the chip results credible. Conclusion Most genes differentially expressed in human traumatic brain cortex selected by gene chip have not been reported in the literature of previous craniocerebral trauma and their intervention is expected to become a new target of gene therapy for traumatic brain injury.