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利用RT -PCR和RACE方法获得了编码荔枝乙烯受体的cDNA序列 ,长度为 2 ,193bp ,该序列中包含一个ORF全长 1,84 8bp ,编码 6 15个氨基酸 ,推测的分子量为 6 8kD。由该序列推定的蛋白质氨基末端存在疏水区 ,羧基端存在组氨酸激酶区和GAF区。与拟南芥的五个乙烯受体比较 ,推定的荔枝乙烯受体的激酶区有乙烯受体所具有的 5个基序 (H ,N ,G1,F ,G2 )中的H和N基序 (motif) ,分别位于第4 2 3- 4 32和 5 32 - 5 4 3氨基酸 ,其保守序列H为 :MNHEMRTPM ;N为 :LMQTILNIMGNA。结构分析和功能预测表明 ,本研究获得的荔枝乙烯受体编码序列推定的氨基酸序列具有乙烯受体的典型特征 ,初步分析表明获得的荔枝cDNA序列包含了编码荔枝乙烯受体 ,LcERS1,的全长cDNA基因 (lcers1) ,GenBank登录号为AY311484。
The cDNA sequence encoding ethylene receptor of lychee was obtained by RT-PCR and RACE. The length of the cDNA is 2,193bp. The ORF contains a full-length ORF of 1,884 bp, encoding 615 amino acids and a predicted molecular weight of 68 kD. The protein deduced from this sequence has a hydrophobic region at the amino terminus and a histidine kinase region and a GAF region at the carboxy terminus. Compared with the five ethylene receptors of Arabidopsis thaliana, the putative ethylene kinase domain of the ethylenediamine receptor has the H and N motifs of the five motifs (H, N, G1, F, G2) possessed by ethylene receptors (motif) located at the 4 2 3 4 32 and 5 32 - 54 3 amino acids, respectively. The conserved sequence H is: MNHEMRTPM; N is: LMQTILNIMGNA. Structural analysis and functional prediction showed that the amino acid sequence deduced from the ethylene receptor coding sequence of the litchi obtained in this study possesses the typical characteristics of the ethylene receptor. Preliminary analysis shows that the obtained litchi cDNA sequence contains the full length of the ethylene receptor LcERS1 cDNA gene (lcers1), GenBank accession number is AY311484.