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目的 研究血脂异常与高血压以及血脂异常合并高血压患者的血管紧张素Ⅱ Ⅰ型受体基因多态性的分布情况。方法 盐析法提取原发性高血压组 (n =130 )和正常对照组 (n =5 10 )的白细胞脱氧核糖核酸 (DNA) ,采用多聚酶链反应 (PCR)结合限制性内切酶方法检测AT1R基因A116 6C多态性 ,并用全自动生化检测仪测定所有研究对象的各项血生化指标。结果 混合性高脂血症患者的AC基因型显著高于正常对照组 (P <0 .0 0 1) ,有统计学意义。合并有高脂血症的高血压患者C等位基因频率显著高于正常对照组 ,统计学分析有意义。结论 AT1R基因A116 6C多态性与血脂异常有关 ,尤其高血压合并血脂异常者关系更加密切。
Objective To study the distribution of angiotensin Ⅱ type 1 receptor gene polymorphism in patients with dyslipidemia and hypertension and dyslipidemia complicated with hypertension. Methods The leukocyte DNA of primary hypertension group (n = 130) and normal control group (n = 5 10) were extracted by salting out method and detected by polymerase chain reaction (PCR) and restriction endonuclease AT1R gene A116 6C polymorphism, and all the biochemical indicators of all subjects were measured by automatic biochemical analyzer. Results AC genotypes in patients with mixed hyperlipidemia were significantly higher than those in normal controls (P <0.01), with statistical significance. The frequency of C allele in hypertensive patients with hyperlipidemia was significantly higher than that in normal control group, and statistical analysis was significant. Conclusion A116 6C polymorphism of AT1R gene is associated with dyslipidemia, especially hypertension with dyslipidemia.