一、引言大肠杆菌的碱性磷酸酯酶是非专一的磷酸单酯酶,是核酸研究的重要工具酶。但是,不含杂酶的碱性磷酸酯酶来之不易,而且反应后为了除去酶往往造成产物得率较低。将酶固定化,则可能解决这些弊病。碱性磷酸酯酶已尝试固定化在多孔玻璃、纤维素或乙烯顺丁烯二酸酐上,它们或是稳定性较差,或是机械性能不适合于装柱连续工作。我们在前文基础 First, the introduction of E. coli alkaline phosphatase is a non-specific phosphomonoesterase, nucleic acid research is an important tool for enzymes. However, alkaline phosphatase which does not contain a heterozygous enzyme is not easy to obtain, and the yield of the product tends to be lower in order to remove the enzyme after the reaction. Immobilization of enzymes may solve these drawbacks. Alkaline phosphatase has been tried to immobilize on porous glass, cellulose or ethylene maleic anhydride, either because of its poor stability or because of its poor mechanical properties for continuous column loading. We are based on the previous article