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应用雌激素受体阳性的人乳腺癌细胞株（ＭＣＦ７细胞），在体外培养条件下，观察三苯氧胺（ＴＡＭ）和重组人白细胞介素６（ＩＬ６）对该细胞凋亡的影响。样品经碘化丙啶（ＰＩ）染色后用ＥＰＩＣＳＥＬＩＴＥ流式细胞仪测定并分析。结果表明，ＴＡＭ和ＩＬ６皆可诱导ＭＣＦ７细胞凋亡，与对照组相比具显著性差异（Ｐ＜０．０１）。若将ＴＡＭ和ＩＬ６联合应用，其诱导ＭＣＦ７细胞凋亡数明显上升，与单药应用比较具非常显著性差异（Ｐ＜０．０１），提示２药合用的效果不是简单的加成而是协同作用。
Using estrogen receptor-positive human breast cancer cell lines (MCF-7 cells), we observed the effects of tamoxifen (TAM) and recombinant human interleukin-6 (IL-6) on apoptosis in vitro. . Samples were stained with propidium iodide (PI) and analyzed by EPICS ELITE flow cytometry. The results showed that both TAM and IL-6 could induce apoptosis of MCF-7 cells, which was significantly different from the control group (P<0.01). If combined with TAM and IL-6, the number of apoptotic cells induced by MCF-7 cells increased significantly, compared with single drug application has a very significant difference (P <0.01), suggesting that the effect of combination of 2 drugs is not a simple plus Instead, they act synergistically.