目的建立射干合剂的液相色谱指纹图谱标准。方法用Inertsil ODS-3色谱柱分离样品,流动相为乙腈-0.5%磷酸(pH 2.25),梯度洗脱,流速1 mL·min~(-1),检测波长215 nm,记录射干合剂的液相色谱指纹图谱。结果射干合剂指纹图谱有17个共有峰,多数峰可以达到良好分离。结论本法具有良好的精密度、稳定性和重复性,指纹图谱的检测标准可以作为射干合剂质量标准。 Objective To establish the standard of liquid chromatography fingerprinting of Shegan Mixture. Methods The sample was separated on an Inertsil ODS-3 column. The mobile phase consisted of acetonitrile-0.5% phosphoric acid (pH 2.25) with gradient elution at a flow rate of 1 mL · min -1 and a detection wavelength of 215 nm. Chromatographic fingerprinting. Results Shegan mixture fingerprints have 17 common peaks, the majority of peaks can achieve good separation. Conclusion The method has good precision, stability and repeatability. The detection standard of fingerprint can be used as the quality standard of Shegan Mixture.