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目的探讨结直肠细胞癌周期蛋白E、生存素及PTEN基因蛋白表达与临床病理特征及预后的相关性。方法采用链霉菌抗生物素蛋白-过氧化酶法(S-P法)检测周期蛋白E、生存素及PTEN基因蛋白在有完整随访资料的69例结直肠癌组织中的表达,并作单因素和多因素生存分析。结果周期蛋白E或PTEN表达与结直肠癌患者年龄、组织学分级及TNM分期有关(P<0.05),生存素表达与组织学分级及TNM分期有关(P<0.05)。周期蛋白E或生存素表达与PTEN表达之间呈显著负相关(P<0.05)。单因素分析显示,患者生存率与年龄、组织学分级、浸润深度、TNM分期及生存素或PTEN的表达均有关(P<0.05)。但经多因素Cox比例风险模型分析,只有组织学分级、TNM分期及PTEN的表达具有独立的预后意义。结论周期蛋白E、生存素及PTEN基因蛋白在结直肠癌中的表达与一些临床病理特征有关。组织学分级为高级别组、TNM分期为Ⅲ、Ⅳ期及PTEN失表达可作为结直肠癌预后不良的独立性指标。
Objective To investigate the correlation between the expression of cyclin E, survivin and PTEN protein and the clinicopathological features and prognosis in colorectal cancer. Methods The expression of cyclin E, survivin and PTEN protein in 69 cases of colorectal cancer with complete follow-up data was detected by streptavidin-peroxidase method (SP method) Factor Survival Analysis. Results The expression of cyclin E or PTEN was related to the age, histological grade and TNM stage (P <0.05). The expression of survivin was correlated with histological grade and TNM stage (P <0.05). Cyclin E or survivin expression was negatively correlated with PTEN expression (P <0.05). Univariate analysis showed that the survival rate was related to age, histological grade, depth of invasion, TNM staging and expression of survivin or PTEN (P <0.05). However, the multi-factor Cox proportional hazards model analysis, only the histological grade, TNM staging and PTEN expression with independent prognostic significance. Conclusion The expressions of cyclin E, survivin and PTEN in colorectal cancer are related to some clinicopathological features. Histological grade was high grade group, TNM stage Ⅲ, Ⅳ and PTEN loss of expression can be used as a marker of poor prognosis of colorectal cancer.