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转绿型叶色突变体是研究植物叶绿体分化与发育的基础材料。grc2是利用60Co-γ射线诱变籼型三系保持系T98B后获得的单叶独立转绿型黄化突变体。grc2植株上任一叶片刚抽出时为黄色,在生长10 d左右后变绿,具有单叶不依赖于植株特定发育阶段而独立转绿的特性。与野生型T98B相比,grc2黄化叶片的总叶绿素和叶绿素b含量显著降低,叶绿体滞留在黄化质体阶段,表明grc2可能在叶片早期发育中起关键作用。遗传分析表明,grc2受1对隐性核基因独立控制;利用源于grc2/Nipponbare的F2群体的960个突变单株,将grc2基因定位在STS标记S254与S258之间约31 kb的范围内,该区域含有5个未报道过的注释基因。这些结果为grc2的克隆及功能研究提供了重要信息。
To green leaf color mutant is to study the plant chloroplast differentiation and development of the basic material. Grc2 is an independent leaf-turning green-yellowing mutant obtained by mutagenesis of indica line T98B with 60Co-γ ray. Any of the leaves on the grc2 plant was yellow just after being extracted, and became green after about 10 days of growth. It has the characteristic that the single leaf does not depend on the specific development stage of the plant and turns green independently. Compared with the wild-type T98B, total chlorophyll and chlorophyll b content of the leaves of grc2 decreased significantly, and chloroplasts retained in the protoplast stage, indicating that grc2 may play a key role in early leaf development. Genetic analysis showed that grc2 was independently controlled by a pair of recessive nuclear genes. Using 960 mutant individuals of F2 population derived from grc2 / Nipponbare, the grc2 gene was located within a range of about 31 kb between STS markers S254 and S258, The area contains five non-reported annotation genes. These results provide important information for the cloning and functional studies of grc2.