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目的:观察葛根素对血管紧张素Ⅱ(AngⅡ)诱导血管平滑肌细胞(VSMC)增殖及对蛋白激酶-α(PKC-α)和核转录因子(NF)-κB表达的影响,探讨其可能存在的抑制VSMC增殖的分子机制。方法:体外培养Wistar大鼠的VSMC,给予单纯1.5×10-3mol/L葛根素处理2 h(P组)、单纯10-8mol/L AngⅡ处理24 h(A组)和1.5×10-3mol/L葛根素预处理2 h后再加10-8mol/L AngⅡ处理24 h(P+A组)刺激后,四氮唑盐法检测增殖情况,采用Western blot的方法检测PKC-α和NF-κB的表达。结果:对照组、P组、A组、P+A组VSMC增殖的A值分别为0.178±0.017、0.198±0.028、0.373±0.017和0.286±0.024。与对照组比较,A组和P+A组差异有统计学意义(均P<0.05);A组和P+A组VSMC中PKCα-、NFκ-B的表达与对照组比较,均显著增高(均P<0.05);P+A组较A组均有明显下降(均P<0.05)。结论:一定浓度的葛根素可通过下调PKCα-和NF-κB的表达来抑制AngⅡ引起的VSMC的增殖。
OBJECTIVE: To observe the effects of puerarin on the proliferation of vascular smooth muscle cells (VSMC) induced by angiotensin II (AngII) and the effects on the expression of protein kinase-α (PKC-α) and nuclear factor-κB (NF-κB), and to explore the possible existence of puerarin. The molecular mechanism of inhibition of VSMC proliferation. METHODS: Wistar rat VSMCs were cultured in vitro and treated with pure 1.5×10-3 mol/L puerarin for 2 h (P group), 10-8 mol/L AngII alone for 24 h (group A) and 1.5×10-3 mol/mL. L-puerarin was pretreated for 2 h and then treated with 10-8 mol/L AngII for 24 h (P+A group). Proliferation was detected by tetrazolium salt method. PKC-α and NF-κB were detected by Western blot. expression. Results: The A values of VSMC proliferation in the control group, P group, A group, and P+A group were 0.178±0.017, 0.198±0.028, 0.373±0.017, and 0.286±0.024, respectively. Compared with the control group, the difference between the A group and the P+A group was statistically significant (all P<0.05); the expression of PKCα- and NFκ-B in the VSMCs of the A group and the P+A group was significantly higher than that of the control group ( All P <0.05); P + A group were significantly lower than A group (all P <0.05). Conclusion: Puerarin at certain concentration can inhibit the proliferation of VSMC induced by AngII by down-regulating the expression of PKCα- and NF-κB.