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研究表明创伤弧菌产生的金属蛋白酶(Vibrio vulnificus protease,VVP)与创伤弧菌的致病性密切相关。经过纯化的VVP能加强血管的渗透性,而且能破坏血管基底膜而导致严重的出血。VVP能使再生基底膜的胶化下降,使出血组织损伤。VVP前体经过水解氨基末端终止信号肽和前肽而成为成熟蛋白酶。成熟的VVP包含两个功能区域,分别是介导蛋白水解反应的氨基末端多肽片段和介导与靶目标有效连接的羧基末端片段。创伤弧菌有LuxS/AI-2和SmcR两种群体感应(Quorum sensing,QS)系统,均能调控蛋白酶的表达,并且二者之间可能存在相互作用或等级反应。LuxS对创伤弧菌的致病力是必需的,但SmcR却是非必需的。
Studies have shown that the Vibrio vulnificus protease (VVP) produced by Vibrio vulnificus is closely related to the pathogenicity of Vibrio vulnificus. Purified VVP can enhance vascular permeability, but also can damage the vascular basement membrane and cause severe bleeding. VVP can make the regeneration of the basement membrane gel decreased bleeding tissue damage. VVP precursors become mature proteases by terminating the signal peptide and propeptide by hydrolyzing the amino terminus. The mature VVP contains two functional regions, namely the amino-terminal polypeptide fragment that mediates the proteolytic reaction and the carboxyl -terminal fragment that mediates the efficient ligation with the target. Vibrio vulnificus has two systems of Quorum sensing (QS), LuxS / AI-2 and SmcR. Both of them can regulate the expression of protease, and there may be interaction or level reaction between them. LuxS is essential for the virulence of Vibrio vulnificus, but SmcR is not necessary.