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为揭示小麦(Triticum aestivumL.)生理型雄性不育机理,以化学杂交剂SQ-1为诱导剂,构建了普通小麦西农1376不育和可育生理系,用RT-PCR技术分析了3-磷酸甘油醛脱氢酶(GAPDH)和NFUDP4基因在不同发育时期的不育和可育花药中的表达差异。结果表明,与同期对照相比,GAPDH和NFUDP4基因在不育花药单核期到三核期均下调表达,其中在大量花粉粒败育的单核期,GAPDH下调表达尤为显著。因此,小麦生理型雄性不育花药败育过程中,一方面由于GAPDH基因表达受抑制,使糖酵解受阻导致能量供应不足;另一方面,NFUDP4基因下调表达,可能引起线粒体某一Fe-S族蛋白装配需要的分子骨架减少而使其数量不足,从而可能引起Fe-S族蛋白参与的生化反应减弱,如呼吸链电子传递受阻引起能量供应不足,与小麦生理型雄性不育具有一定关系。
In order to reveal the mechanism of physiological male sterility in wheat (Triticum aestivum L.), a chemical hybridization reagent SQ-1 was used as an inducer to construct the sterile and fertile physiological lines of common wheat Xinong 1376. The effects of 3-phosphate Differences in expression of glyceraldehyde dehydrogenase (GAPDH) and NFUDP4 in sterile and fertile anther at different developmental stages. The results showed that compared with the same period, GAPDH and NFUDP4 genes were down-regulated in the mononuclear to trinucleate stages of the anthers, especially in the mononuclear stage of many pollen abortion, the expression of GAPDH was down-regulated significantly. Therefore, during the abortion of male sterile physiological anthers of wheat, on the one hand, the expression of GAPDH gene is inhibited and the energy supply is insufficient due to the block of glycolysis; on the other hand, the down-regulation of NFUDP4 gene may cause some Fe-S The reduction of the molecular skeleton required by the assembly of the family of proteins results in an insufficient number of molecular skeletons, which may cause biochemical reactions involving Fe-S proteins to be weakened. For example, insufficient energy supply due to the blocked electron transport in the respiratory chain has some relations with the physiological male sterility of wheat.