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目的:研究白杨素(ChR)是否具有增敏重组人可溶性肿瘤坏死因子相关凋亡诱导配体(rhsTRAIL)诱导人肝癌Bel-7402细胞凋亡作用。方法:体外培养人肝癌Bel-7402细胞。碘化丙啶(PI)染色流式细胞术(FCM)分析细胞死亡率。DNA琼脂糖凝胶电泳确证诱导细胞凋亡作用。Western Bloting检测细胞DR5蛋白的表达。结果:ChR40μmol/L、rhsTRAIL 100ng/mL以及两者合用的细胞死亡率分别为4.91%±0.38%、5.89%±0.39%和28.7%±2.50%。ChR(40μmol/L)联合rhsTRAIL(100ng/mL)处理48小时,人肝癌Bel-7402细胞展示出典型DNA梯形条带图谱。Western Blot分析结果发现:白杨素以浓度和时间依赖的方式上调Bel-7402细胞DR5表达。结论:亚细胞毒性浓度的白杨素具有敏化rhsTRAIL诱导人肝癌Bel-7402细胞凋亡作用。
OBJECTIVE: To investigate whether chrysene (ChR) can sensitize human hepatocellular carcinoma Bel-7402 cells to apoptosis induced by recombinant human soluble tumor necrosis factor-related apoptosis-inducing ligand (rhsTRAIL). Methods: Human hepatoma Bel-7402 cells were cultured in vitro. Propidium iodide (PI) staining flow cytometry (FCM) was used to analyze cell death rates. DNA agarose gel electrophoresis to confirm the induction of apoptosis. Western Bloting was used to detect the expression of DR5 protein. Results: The combined cell death rates of ChR40μmol / L, rhsTRAIL 100ng / mL and their combination were 4.91% ± 0.38%, 5.89% ± 0.39% and 28.7% ± 2.50% respectively. After treatment with ChR (40μmol / L) and rhsTRAIL (100ng / mL) for 48 hours, Bel-7402 cells showed a typical DNA ladder pattern. Western Blot analysis showed that chrysin could up-regulate the expression of DR5 in Bel-7402 cells in a concentration-and time-dependent manner. CONCLUSIONS: Chrysin with sub-cytotoxicity has sensitized rhsTRAIL-induced apoptosis in human hepatoma Bel-7402 cells.