论文部分内容阅读
目的探讨浆细胞膜糖蛋白1(PC-1)基因K121Q多态性与2型糖尿病及糖尿病合并冠状动脉粥样硬化性心脏病(冠心病)的关系。方法应用聚合酶链反应-限制性酶切片段长度多态性分析(PCR-RFLP)技术检测糖尿病(DM组,n=80)及其合并冠心病患者(DC组,n=67)与健康对照组(n=83)的PC-1基因K121Q的多态性分布,比较各组间基因型、等位基因分布和频率及相关临床资料。结果 DM组KQ+QQ基因型频率和Q等位基因频率高于健康对照组(P<0.05)。DC组中KQ基因携带者为64.2%,而DM组中KQ基因型携带者为41.3%,两组差异有统计学意义(P<0.05)。在DC组和DM组中KQ+QQ基因型患者的数量是KK基因型的3.0倍。结论携带Q等位基因者对2型糖尿病的易感性增强,且更易患冠心病。
Objective To investigate the relationship between plasma membrane glycoprotein 1 (PC-1) gene K121Q polymorphism and type 2 diabetes mellitus and diabetic patients with coronary heart disease (coronary heart disease). Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect the expression of MMP-2 in diabetes mellitus (DM group, n = 80) and its patients with coronary heart disease (DC group, n = 67) Group (n = 83) PC-1 gene K121Q polymorphism distribution between the groups genotypes, allele distribution and frequency and related clinical data. Results The frequencies of KQ + QQ genotype and Q allele in DM group were higher than those in healthy control group (P <0.05). The percentage of KQ gene carriers in DC group was 64.2%, while that in DM group was 41.3%. There was significant difference between the two groups (P <0.05). The number of patients with KQ + QQ genotype in the DC and DM groups was 3.0 times that of the KK genotype. CONCLUSIONS: Q-allele carriers are more susceptible to type 2 diabetes and are more susceptible to coronary heart disease.