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目的观察和分析Epstein-Barr病毒(EBV)感染传染性单核细胞增多症(IM)患儿血浆可溶性人类白细胞抗原G(s HLA-G)水平与外周血淋巴细胞亚群的关系。方法选取90例EBV感染IM患儿作为病例组,选取90例健康儿童作为对照组,对两组患者血浆s HLA-G水平和外周血淋巴细胞亚群进行检测和比较。结果病例组患儿的血浆s HLA-G水平显著高于对照组儿童(Z=59.128,P<0.05);病例组患儿的外周血CD3+淋巴细胞、CD8+淋巴细胞的百分比均显著高于对照组(t=10.365、19.205,P<0.05),而其外周血CD4+淋巴细胞、CD16+56+淋巴细胞、CD19+淋巴细胞的百分比及CD4+/CD8+淋巴细胞比值均显著低于对照组(t=12.183、4.358、8.138、10.226,P<0.05);患儿血浆s HLA-G水平与其外周血淋巴细胞亚群指标均无相关性(P>0.05)。结论 EBV感染IM患儿表现为血浆s HLA-G高表达和外周血淋巴细胞亚群紊乱,患儿机体内存在着明显的免疫耐受和免疫抑制,但s HLA-G高表达并不是引起患儿免疫功能下降的直接原因。
Objective To observe and analyze the relationship between plasma soluble human leukocyte antigen G (s HLA-G) and peripheral blood lymphocyte subsets in Epstein-Barr virus (EBV) infected infectious mononucleosis (IM). Methods 90 cases of EBV-infected children with IM were selected as the case group and 90 healthy children were selected as the control group. Plasma s HLA-G levels and peripheral blood lymphocyte subsets were detected and compared in two groups. Results The plasma levels of s HLA-G in children with the disease were significantly higher than those in the control group (Z = 59.128, P <0.05). The percentage of CD3 + lymphocytes and CD8 + lymphocytes in the peripheral blood of the children in the case group were significantly higher than those in the control group (t = 10.365,19.205, P <0.05). The percentage of CD4 + lymphocytes, CD16 + 56 + lymphocytes, CD19 + lymphocytes and CD4 + / CD8 + lymphocytes in peripheral blood were significantly lower than those in control group (t = 4.358, 8.138, 10.226, P <0.05). There was no correlation between the levels of s-G in plasma and peripheral blood lymphocyte subsets (P> 0.05). Conclusions The expression of HLA-G in peripheral blood and the abnormality of peripheral blood lymphocyte subsets in EBV-infected IM patients are obvious. There is obvious immune tolerance and immunosuppression in children with EBV infection, but high HLA-G expression is not caused by The direct cause of the decline in immune function.