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【目的】ClfA蛋白是金黄色葡萄球菌粘附于宿主细胞的最主要粘附因子之一,筛选鉴定ClfA蛋白的Th表位,研制基于表位的联合疫苗将成为该菌疫苗新的发展方向。【方法】本研究采用生物信息学软件预测ClfA可能的H-2d限制性Th表位,并以CD4+T淋巴细胞增殖实验及流式细胞分析进行筛选和鉴定。【结果】获得BALB/c小鼠H-2d限制性Th1表位(C335)和Th2表位(C214,C286和C436)。【结论】筛选出优势性Th细胞表位,并对表位的免疫学特性进行实验免疫研究,为最终研制保护作用强、安全性高的新型疫苗奠定基础。
【Objective】 ClfA protein is one of the most important adhesion molecules for Staphylococcus aureus to adhere to host cells. Screening and identifying the Th epitope of ClfA protein and developing a vaccine based on epitopes will be a new development direction of the vaccine. 【Methods】 In this study, bioinformatics software was used to predict the possible H-2d-restricted Th epitope of ClfA, and its screening and identification were performed by CD4 + T lymphocyte proliferation assay and flow cytometry analysis. [Results] The H-2d restricted Th1 epitope (C335) and Th2 epitope (C214, C286 and C436) of BALB / c mice were obtained. 【Conclusion】 The dominant Th cell epitopes were screened and the immunological characteristics of the epitopes were studied by immunological experiments. This will lay the foundation for the development of a new type of vaccine with strong protection and high safety.