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目的观察血管紧张素ⅡⅠ型受体(ATI)拮抗剂缬沙坦对兔动脉成形术后血管内膜增生及内皮细胞CD34表达的影响,探讨ATI受体拮抗剂治疗血管再狭窄的机制和作用。方法雄性新西兰白兔24只,随机分成3组:对照组始终以普通饲料饲养12周,不加任何处理;模型组和缬沙坦给予高胆固醇喂养,4周后行腹主动脉内膜剥脱,继续高胆固醇饮食4周后行球囊成形术,模型组继续普通饲料饲养,而缬沙坦组给普通饲料+缬沙坦10mg·kg~(-1)·d~(-1)喂养4周。12周末取腹主动脉行病理形态学观察及CD34免疫组织化学分析。结果与模型组比较,缬沙坦组内膜厚度减少56.58%,内膜面积减少66.81%。免疫组织化学分析显示,缬沙坦组与模型组相比CD34表达增加(P<0.01)。结论缬沙坦可以减轻兔腹主动脉成形术后内膜增生,其机制可能与促进内膜损伤后的内皮细胞增殖,提高内皮修复功能有关。
Objective To investigate the effect of valsartan, an antagonist of angiotensin Ⅱ type 1 receptor (ATI), on intimal hyperplasia and endothelial CD34 expression after angioplasty in rabbits and to explore the mechanism and effect of ATI receptor antagonist on vascular restenosis. Methods Twenty-four male New Zealand white rabbits were randomly divided into three groups: the control group was fed with normal feed for 12 weeks without any treatment; the model group and valsartan were given high cholesterol; the abdominal aortic endarterectomy was continued after 4 weeks, Balloon angioplasty was performed 4 weeks after the cholesterol diet. The model group was fed with normal feed while the valsartan group fed with normal diet + valsartan 10 mg · kg -1 d -1 for 4 weeks. The pathological morphology and CD34 immunohistochemical analysis of the abdominal aorta were taken at 12 weeks. Results Compared with the model group, the intima thickness of valsartan group decreased 56.58% and the intima area decreased 66.81%. Immunohistochemical analysis showed that CD34 expression was increased in valsartan group compared with model group (P <0.01). Conclusion Valsartan attenuates neointimal hyperplasia after aortic valve angioplasty in rabbits. The mechanism may be related to the promotion of endothelial cell proliferation and endothelial repair after intimal injury.