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香荚兰的顶芽在MS+BA0.2亳克/升+NAA1.0毫克/升或1/2MS+AB0.5亳克/升的培养基上培养,丛芽分化快,频率高,平均每6周可繁殖一代,具有良好的分化效果。继代培养一年,增殖未见下降。使用1/2MS+NAA0.3亳克/升的培养基单株培养,可促进生根,培育壮苗。待苗高10—15厘米,叶子充分舒展即可移植于疏松通气的草炭土加炭碎上。为解决育种和良种的快速繁殖提供了新的手段。
The top buds of vanilla were cultured on medium with MS + BA0.2 mg / L + NAA1.0 mg / L or 1/2 MS + AB0.5 mg / L, with fast clustering, high frequency and average Breeding a generation every 6 weeks, with good differentiation. Subculture for one year, no decline in proliferation. The use of 1 / 2MS + NAA0.3 mg / liter of culture medium, can promote rooting, nurturing seedlings. To be 10-15 cm seedling height, the leaves can be fully stretched and transplanted in loose aerated charcoal soil plus carbon broken. It has provided new means to solve the breeding and the rapid propagation of seed.