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[目的]恒河猴(Macaca mulatta)是异种移植研究和药物临床前评价中最重要的动物模型。为了探索临床前研究与临床应用的差距的可能原因,初步研究了恒河猴的血清蛋白质。[方法]分别采集36只正常恒河猴(成都平安动物繁育基地)、30只正常香猪(贵州大学农学院养殖场)和46个正常人(华西医院)的静脉血,采用REP电泳系统电泳血清蛋白。结果采用SPSS 12.0统计学软件分析。[结果]猴血清蛋白中的Alb百分含量比人的低、而比猪的高(P﹤0.05),Alpha1、Alpha2百分含量比人的高、而比猪的低(P﹤0.05),Beta百分含量比猪和人的都低(P﹤0.05),Gamma百分含量比猪的低(P﹤0.05)。[结论]结果显示了猴与人、猪在血清蛋白组分上都存在着一定的差异。这些差异可能会致在猴体内进行的药物临床前研究结果与在人体内的真实情况不符,从而影响药物的临床应用价值和安全性评价;也可能是猪到猴或猪到人的异种移植中生物功能不匹配的潜在原因,而猴和人的差异也可能影响异种移植后的监测和对排斥强度的估计。
[Objective] Macaca mulatta is the most important animal model in xenotransplantation and preclinical evaluation of drugs. In order to explore the possible reasons for the gap between pre-clinical research and clinical application, serum proteins of rhesus monkeys were preliminary studied. [Methods] The venous blood samples of 36 normal rhesus monkeys (Chengdu Ping’an Animal Breeding Base), 30 normal pigs (Guizhou Agricultural College farms) and 46 normal people (West China Hospital) were collected and analyzed by REP electrophoresis system Serum protein. Results using SPSS 12.0 statistical software analysis. [Results] The percentage of Alb in monkey serum protein was lower than that of human and higher than that of pigs (P <0.05). The Alpha1 and Alpha2 percentages were higher than those of humans and lower than those of pigs (P <0.05) Beta percentage was lower in pigs than in pigs (P <0.05), and Gamma percentage was lower in pigs than in pigs (P <0.05). [Conclusion] The results showed that there were some differences in serum protein between monkeys and pigs. These differences may lead to preclinical studies of drugs in the monkey do not match the real situation in the human body, thus affecting the clinical value of the drug and safety evaluation; may also be pig to monkey or pig to human xenografts Potential causes of mismatch in biological function, and differences between monkeys and humans may also influence post-xenographic monitoring and estimation of the intensity of repulsion.