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目的:建立锦灯笼宿萼的含量测定。方法:采用HPLC法测定锦笼灯醇提物不同洗脱部位中4,7—二脱氢新酸浆苦素B的含量,采用C18柱;甲醇-0.025mol/L磷酸水(40:60);检测波长为284 nm。结果:线性范围在0.84~4.2μg(r=0.9994);加样回收率99.8%(n=5)。结论:方法稳定性、重复性好,结果准确、可靠。
Objective: To establish the content determination of Jin lantern lanterns. METHODS: HPLC method was used to determine the content of 4,7-didehydroquinoin B in different elution sites of the ethanol extract of C. napus, using C18 column; methanol-0.025 mol/L phosphoric acid water (40:60) Detection wavelength is 284 nm. Results: The linear range was 0.84~4.2μg (r=0.9994); the recovery rate was 99.8% (n=5). Conclusion: The method has good stability and repeatability, and the result is accurate and reliable.