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目的探讨不同浓度异氟醚预处理延迟相对兔心肌缺血再灌注损伤的影响。方法30只健康新西兰雄性大白兔随机分成三组:1组:缺血再灌注组,行冠状动脉左前降支阻断40 min,再灌注120 min;2组:1.0%异氟醚预处理组,予以1.0%异氟醚+100%氧气持续吸入2 h后停止吸入异氟醚,于24 h后同1组处理;3组:2.0%异氟醚预处理组,予以2.0%异氟醚+100%氧气持续吸入2 h后停止吸入异氟醚,于24 h后同2组处理。各组分别于左冠前降支阻断前20 min(T1)、左冠前降支阻断20 min(T2)、左冠前降支阻断40 min(T3)、心肌再灌注1 h(T4)心肌再灌注2 h(T5)五个时点取颈内动脉血测定肌钙蛋白(cTnI)浓度。再灌注结束后观察心肌超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及心肌细胞超微结构的变化。用伊文思蓝和TTC染色法测心梗面积,同时观察心肌细胞超微结构变化。结果①2组和3组肌钙蛋白增高程度低于1组(P<0.05)。②心肌梗死面积:2组(24.2%±2.1%)和3组(19.7%±2.8%)低于1组(37.8%±1.7%)(P<0.05)。③心肌细胞电镜观察显示预处理组心肌细胞损伤轻于对照组。④2组和3组MDA较1组降低(P<0.05),SOD较1组增高(P<0.05)。结论异氟醚预处理延迟相对兔心肌缺血再灌注损伤有保护作用。
Objective To investigate the effects of different concentrations of isoflurane preconditioning on myocardial ischemia-reperfusion injury in rabbits. Methods Thirty healthy male New Zealand white rabbits were randomly divided into three groups: Group 1: Ischemia / reperfusion group, the left anterior descending coronary artery was occluded for 40 min and reperfused for 120 min; Group 2: 1.0% isoflurane preconditioning group, Isoflurane + 100% oxygen was inhaled with 1.0% oxygen for 2 h, then inhaled isoflurane, and treated with 1 group after 24 h; 3 groups: 2.0% isoflurane pretreatment group, 2.0% isoflurane +100 After continuous inhalation of oxygen for 2 h, inhalation of isoflurane was stopped and treated with 2 groups after 24 h. The rats in each group were divided into two groups: 20 min before the left anterior descending coronary artery occlusion (T1), 20 min before the left anterior descending coronary artery occlusion (T2), 40 min (T3) T4) at 2 hours after myocardial reperfusion (T5), the concentration of troponin (cTnI) was measured by internal carotid artery. Myocardial superoxide dismutase (SOD) activity, malondialdehyde (MDA) content and myocardial ultrastructure were observed after reperfusion. The area of myocardial infarction was measured by Evans Blue and TTC staining, and the changes of myocardial ultrastructure were also observed. Results ① The levels of troponin in groups 2 and 3 were lower than those in group 1 (P <0.05). ② The area of myocardial infarction was lower in group 2 (24.2% ± 2.1%) and group 3 (19.7% ± 2.8%) than in group 1 (37.8% ± 1.7%) (P <0.05). ③ Electron microscopy of myocardial cells showed preconditioning cardiomyocytes injury lighter than the control group. ④ MDA in group 2 and group 3 was lower than that in group 1 (P <0.05), and SOD was higher than that in group 1 (P <0.05). Conclusion Delayed isoflurane preconditioning has a protective effect on myocardial ischemia-reperfusion injury in rabbits.