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目的建立三七花蕾中8种四环三萜皂苷(人参皂苷Rg1、Re、Rb1、Rc、Rb2、Rb3、Rd和三七皂苷R1)的高效液相色谱定性定量测定方法。方法采用Hypersil ODS(4.6 mm×250 mm,5μm)分析柱,以含0.005%甲酸的乙腈-水进行梯度洗脱,在65 min内获得良好分离。结果三七花蕾中8种皂苷在一定的浓度范围内,与峰面积呈良好的线性关系,回收率分别为:R195.6%~102.5%(n=3),Rg198.8%~103.1%(n=3),Re 95.3%~101.7%(n=3),Rb198.9%~101.1%(n=3),Rc95.6%~102.3%(n=3),Rb299.0%~100.4%(n=3),Rb399.2%~100.3%(n=3),Rd 95.0%~96.8%(n=3)。结论该方法灵敏快速,准确可靠,可用于三七花蕾的质量控制。
Objective To establish a qualitative and quantitative HPLC method for the determination of eight tetracycline triterpene saponins (ginsenosides Rg1, Re, Rb1, Rc, Rb2, Rb3, Rd and notoginsenoside R1) in the buds of Panax notoginseng. Methods A Hypersil ODS (4.6 mm×250 mm, 5 μm) analytical column was used. The gradient elution was performed with acetonitrile-water containing 0.005% formic acid and a good separation was achieved within 65 min. Results The eight saponins in the buds of Panax notoginseng showed a good linear relationship with the peak area within a certain concentration range. The recoveries were: R195.6%~102.5% (n=3), Rg198.8%~103.1% ( n=3), Re 95.3%~101.7% (n=3), Rb198.9%~101.1% (n=3), Rc95.6%~102.3% (n=3), Rb299.0%~100.4% (n=3), Rb399.2%~100.3% (n=3), Rd 95.0%~96.8% (n=3). Conclusion The method is sensitive, rapid, accurate and reliable and can be used for the quality control of buds of Panax notoginseng.