目的:建立同时测定黄芩、白芍药对提取物中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、芍药苷、芍药内酯苷、没食子酸和12,,3,4,6-五没食子酰葡萄糖8种成分含量的HPLC方法。方法:Agilent ZORBAXSB-C18色谱柱(150 mm×4.6 mm5,μm),流动相组成A:乙腈,B:0.1%磷酸水溶液,梯度洗脱;流速:1.0 mL.min-1,检测波长:230 nm,柱温:30℃。结果:黄芩苷线性范围为186.4～932.0μg,定量下限为186.4μg(r=0.999 8),平均回收率为100.1%(RSD=2.0%,n=6);黄芩素线性范围为19.12～95.60μg,定量下限为19.12μg(r=0.999 7),平均回收率为99.3%(RSD=2.5%,n=6);汉黄芩苷线性范围为44.20～221.0μg,定量下限为44.20μg(r=0.999 5),平均回收率为98.6%(RSD=2.5%,n=6);汉黄芩素线性范围为10.30～51.50μg,定量下限为10.30μg(r=0.999 9),平均回收率为97.5%(RSD=1.4%,n=6);芍药苷线性范围为4.312～43.12μg,定量下限为4.312μg(r=0.999 6),平均回收率为98.4%(RSD=2.4%,n=6);芍药内酯苷线性范围为3.060～30.60μg,定量下限为3.060μg(r=0.999 5),平均回收率为98.2%(RSD=2.0%,n=6);没食子酸线性范围为9.994～199.9μg,定量下限为9.994μg(r=0.999 5),平均回收率为98.8%(RSD=2.1%,n=6);1,2,3,4,6-五没食子葡萄糖线性范围为8.800～44.00μg,定量下限为8.800μg(r=0.999 5),平均回收率为98.0%(RSD=2.2%,n=6)。结论:本方法可应用于黄芩、白芍药对提取物中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、芍药苷、芍药内酯苷、没食子酸和1,2,3,4,6-五没食子酰葡萄糖8种成分含量的同时测定。 OBJECTIVE: To establish a method for the simultaneous determination of baicalin, baicalein, baicalin, wogonin, paeoniflorin, paeoniflorin, gallic acid and 12,3,4,6 - HPLC method for the content of 8 components of glucose. Methods: Agilent ZORBAXSB-C18 column (150 mm × 4.6 mm 5, μm), mobile phase A: acetonitrile, B: 0.1% phosphoric acid aqueous solution, gradient elution; flow rate: 1.0 mL.min-1; detection wavelength: 230 nm Column temperature: 30 ° C. Results: The linear range of baicalin was 186.4-932.0μg, the lower limit of quantitation was 186.4μg (r = 0.999 8), the average recovery was 100.1% (RSD = 2.0%, n = 6). The linear range of baicalein was 19.12-95.60μg , The lower limit of quantitation was 19.12μg (r = 0.999 7), the average recovery was 99.3% (RSD = 2.5%, n = 6); the linear range of the baicalin was 44.20 ~ 221.0μg and the limit of quantification was 44.20μg The average recovery was 98.6% (RSD = 2.5%, n = 6). The linear range of wogonin was 10.30-51.50μg with the lower limit of quantitation of 10.30μg (r = 0.999 9). The average recovery was 97.5% (RSD = 1.4%, n = 6). The linear range of paeoniflorin was 4.312 ~ 43.12μg and the lower limit of quantitation was 4.312μg (r = 0.999 6). The average recovery was 98.4% (RSD = 2.4%, n = 6) The linear range was 3.060 ~ 30.60μg, the lower limit of quantification was 3.060μg (r = 0.999 5), the average recovery was 98.2% (RSD = 2.0%, n = 6); the linear range of gallic acid was 9.994 ~ 199.9μg, The lower limit of quantitation was 9.994μg (r = 0.999 5) with an average recovery of 98.8% (RSD = 2.1%, n = 6). The linear range of 1,2,3,4,6- The lower limit of quantification was 8.800 μg (r = 0.999 5) with an average recovery of 98.0% (RSD = 2.2%, n = 6). Conclusion: The method can be applied to Scutellaria baicalensis and Paeonia lactiflora on the extraction of baicalin, baicalein, baicalin, wogonin, paeoniflorin, paeoniflorin, gallic acid and 1,2,3,4,6- Simultaneous Determination of Eight Components in Five Galloylglucose.