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本文应用互隔交链孢霉261株提取物261—B_2—3诱发人羊膜FL细胞UDS,以研究其致突变性。按余应年等的方法,在~(14)C—TdR预标记的FL细胞中由提取物引起酸不溶性组分的HU抗性非程序性~3H—TdR掺入。用液闪计数器测量。以放射比~3H/~(14)C为UDS水平的反
In this study, UTS of human amnion FL cells was induced by 261 strains of B. aeruginosa extracts B2-3 to study its mutagenicity. According to Yu Yingnian et al., HU-resistant non-programmative ~3H-TdR incorporation of acid insoluble fractions was induced in ~(14)C-TdR pre-labeled FL cells by extracts. Measured with a liquid flash counter. The radiation ratio ~3H/~(14)C is the inverse of the UDS level.