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目的 以肝癌病人树突状细胞 (DC)体外诱导抗肝癌免疫。方法 自肝癌病人外周血中分离出单个核细胞 (PBMC) ;以人肝癌细胞系HepG2肿瘤细胞的肿瘤相关抗原 (TAA)激活DC ;以粒 巨噬细胞集落刺激因子 (GM CSF)及白介素 4(IL 4 )联合刺激PBMC中DC ;DC诱导自体T淋巴细胞增殖、分化为细胞毒性T细胞 (CTL) ;检测CTL及其上清液对HepG2、BEL 740 2、LOVO及HOS 86 0 3等肿瘤细胞的细胞毒作用。结果 肝癌病人DC能够诱导自体T淋巴细胞增殖、分化为CTL ,该CTL对HepG2肿瘤细胞有强大的杀伤力 ,杀伤率为 (92± 1 0 5 ) % ,对BEL 740 2肿瘤细胞亦有较强的杀伤作用 ,杀伤率为 (6 3± 1 1 ) % ,对LOVO及HOS 86 0 3肿瘤细胞则无明显细胞毒作用 ,杀伤率分别为 (9±5 3) %、(8± 4 2 ) %。结论 肝癌病人外周血DC体外能够诱导高效而特异的抗肝癌免疫。提示DC可能在治疗肝癌及预防肝癌复发和转移中发挥重要作用。
Objective To induce anti-hepatocellular carcinoma immunity in vitro in dendritic cells (DC) of liver cancer patients. Methods Mononuclear cells (PBMCs) were isolated from peripheral blood of patients with hepatocellular carcinoma; DCs were activated by tumor-associated antigen (TAA) of human hepatoma cell line HepG2 tumor cells; granulocyte macrophage colony-stimulating factor (GM CSF) and interleukin 4 ( IL 4 ) co-stimulated DCs in PBMCs; DCs induced proliferation of autologous T lymphocytes and differentiated into cytotoxic T cells (CTLs); CTLs and their supernatants were tested for HepG2, BEL 740 2, LOVO and HOS 86 0 3 and other tumor cells. The cytotoxic effect. Results The DCs of HCC patients could induce the proliferation and differentiation of autologous T lymphocytes into CTLs. The CTLs had a strong lethal effect on HepG2 tumor cells with a killing rate of (92±105) %. They also had strong effects on BEL 740 2 tumor cells. The killing effect was (6 3 ± 1 1) %, and there was no significant cytotoxicity on LOVO and HOS 86 0 3 tumor cells. The killing rates were (9 ± 53)% and (8 ± 4 2), respectively. %. Conclusion Peripheral blood DCs of liver cancer patients can induce efficient and specific anti-hepatocellular carcinoma immunity in vitro. It is suggested that DC may play an important role in the treatment of liver cancer and prevention of recurrence and metastasis of liver cancer.