Objective:To investigate the relationship between tissue distributions of modified Wuzi Yanzong prescription(加味五子衍宗方,MWP) in rats and meridian tropism theory.Methods:A high-performance liquid chromatography with Fourier transform-mass spectrometry (HPLC-FT) method was used to identify the metabolites of MWP in different tissues of rats after continued oral administration of MWP for 7 days.The relationship between MWP and meridian tropism theory was studied according to the tissue distributions of the metabolites of MWP in rats and the relevant literature.Results:Nineteen metabolites,mainly flavanoid compounds,were detected in the different rat tissues and classified to each herb in MWP.Further,it was able to establish that the tissue distributions of the metabolites of MWP were consistent with the descriptions of meridian tropism of MWP available in literature,this result might be useful in clarifying the mechanism of MWP on meridian tropism.In the long run,these data might provide scientific evidence of the meridian tropism theory to further promote the reasonable,effective utilization,and modernization of Chinese medicine.Conclusion:The tissue distributions of MWP in vivo were consistent with the descriptions of meridian tropism of MWP.