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为了实现木质纤维素浓醪酶解在低酶载量时的“三高”(高浓度、高转化率和高转化效率),通过利用常压甘油有机溶剂预处理甘蔗渣为底物,筛选合适的基质质量浓度(150 g/L)、纤维素酶添加量(6 FPU/g基质)和添加剂(吐温80,30 mg/g基质)。接着采用分批补料策略使基质质量浓度达到350 g/L,考察了不同加酶方式对分批补料浓醪酶解的影响。酶解72 h酶解液葡萄糖质量浓度达到132 g/L,葡萄糖转化率达到了理论值的60%。结果表明,常压甘油有机溶剂预处理基质具有较好的可酶解性,添加吐温80可以显著提高酶解效率。常压甘油有机溶剂预处理甘蔗渣的分批补料浓醪酶解推动了纤维素乙醇浓醪发酵工业化进程。
In order to achieve the lignosulphonase hydrolysis at low enzyme loading “three high ” (high concentration, high conversion and high conversion efficiency), through the use of atmospheric glycerol organic solvent pretreatment of bagasse as a substrate, The appropriate substrate concentration (150 g / L), cellulase loading (6 FPU / g substrate) and additives (Tween 80, 30 mg / g substrate) were screened. Then the fed-batch strategy was adopted to make the substrate concentration reach 350 g / L. The effects of different adding methods on the enzymolysis of fed-batch mash were investigated. After 72 h enzymolysis, the glucose concentration reached 132 g / L and the glucose conversion rate reached 60% of the theoretical value. The results showed that the organic solvent pretreated with glycerol under normal pressure had better enzymatic digestibility, and the addition of Tween 80 could significantly improve the enzymolysis efficiency. Atmospheric pressure glycerol organic solvent pretreatment of bagasse batch fed concentrated mash enzymatic promote cellulose ethanol fermentation mash industrialization process.