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目的:探讨肿瘤相关基因ER-α、RAR-β、MGMT及P16INK4a启动子在口腔鳞状细胞癌(OSCC)组织中的甲基化状态。方法:20例病理确诊为OSCC的组织切片,经酶消化法提取组织DNA后双硫法检测ER-α、RAR-β、MGMT及P16INK4a基因启动子的甲基化状态,比较分析4种基因启动子甲基化状态和临床病理参数的相关性。结果:20例中,P16INK4a、MGMT启动子甲基化发生率均为10%,RAR-β启动子甲基化发生率为40%,ER-α启动子甲基化发生率为55%,两株OSCC细胞系中,ER-α、RAR-β启动子均出现甲基化,而MGMT及P16INK4a启动子均未见甲基化。结论:RAR-β、ER-α基因启动子的甲基化较P16INK4a、MGMT g更为常见,提示前两者可能在OSCC的发生中具有更重要的作用。
Objective: To investigate the methylation status of tumor-related genes ER-α, RAR-β, MGMT and P16INK4a in oral squamous cell carcinoma (OSCC). Methods: 20 cases of pathological diagnosis of OSCC tissue sections were digested by enzymatic digestion DNA methylation status detection of ER-α, RAR-β, MGMT and P16INK4a gene promoter, comparative analysis of four genes start Correlation between sub-methylation status and clinicopathological parameters. Results: The methylation rates of P16INK4a and MGMT promoters were all 10%, the methylation of RAR-β promoter was 40% and the methylation of ER-α promoter was 55% Strain OSCC cell lines, ER-α, RAR-β promoter were methylated, while neither MGMT nor P16INK4a promoter methylation. CONCLUSION: Methylation of RAR-β and ER-α gene promoters is more common than P16INK4a and MGMT g, suggesting that the former two may play a more important role in the pathogenesis of OSCC.