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本文对6株恶性疟原虫的特征,包括对培养的适应性,在体外产生配子体的能力以及在培养的前后它们对氯喹的敏感性等进行了研究。虫株的分离采取改良的蜡烛缸技术,转种培养后96小时如果原虫血症增加10倍者视为适应体外培养。配子体血症以检查每100个感染红细胞中的配子体数表示。并以体外微量试验法测定这些虫株对氯喹的敏感性。结果,FAN-5株及FRN-1株恶性疟原虫易于适应体外培养。前者对氯喹始终保持敏感,但不产生明显的配子体血症。相反,
The characteristics of 6 P. falciparums were studied, including their suitability for culture, their ability to produce gametophytes in vitro and their sensitivity to chloroquine both before and after cultivation. Isolation of insect strains to take a modified candle jar technology, 96 hours after the transplanted culture if the parasitemia increased by 10 times as adapted to in vitro culture. Gametogenesis is measured as the number of gametes per 100 infected erythrocytes. The sensitivity of these strains to chloroquine was determined by in vitro micro assay. As a result, FAN-5 and FRN-1 strains of P. falciparum were easily adapted to in vitro culture. The former is always sensitive to chloroquine, but does not produce significant gametophytemia. in contrast,