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目的分析不同年龄呼吸道感染患儿肺炎支原体(Mycoplasma pneumonia,MP)-IgM抗体、MP-DNA检测结果。方法 1 054例呼吸道感染患儿按年龄分为1个月~3岁组586例,>3~6岁组297例,>6~14岁组171例,采用被动凝集法检测3组血清MP-IgM抗体,荧光探针PCR法检测3组呼吸道分泌物咽拭子标本MP-DNA,比较3组MP-IgM抗体、MP-DNA阳性率。结果 1 054例患儿MP-DNA阳性率为15.9%,MP-IgM阳性率12.2%,二者比较差异无统计学意义(P>0.05);1个月~3岁组、>3~6岁组、>6~14岁组MP-IgM抗体阳性率分别为6.3%、48.5%、21.6%,MP-DNA阳性率分别为13.2%、17.2%、23.4%;1个月~3岁组MP-IgM阳性率低于同组MP-DNA阳性率(P<0.05),且低于>3~6岁组、>6~14岁组MP-IgM阳性率(P<0.05)。结论不同年龄儿童免疫系统发育处于不同阶段,对MP免疫应答可能存在差异,应根据具体情况合理选择检测手段,及早明确诊断。
Objective To analyze the detection results of Mycoplasma pneumonia (MP) -IgM antibody and MP-DNA in children with respiratory tract infection of different ages. Methods One hundred and fifty-four children with respiratory tract infection were divided into two groups according to age, 586 cases of 1 month to 3 years old, 297 cases of> 3 to 6 years old and 171 cases of> 6 to 14 years old. The levels of MP- IgM antibody and fluorescent probe PCR were used to detect MP-DNA in throat swab specimens of three groups of respiratory secretions. The positive rate of MP-DNA was compared between the three groups of MP-IgM antibodies. Results The positive rate of MP-DNA in 1 054 children was 15.9%, the positive rate of MP-IgM was 12.2%, there was no significant difference between the two groups (P> 0.05); 1 month to 3 years old> 3-6 years old The positive rates of MP-IgM in group> 6-14 years old were 6.3%, 48.5% and 21.6%, respectively. The positive rates of MP-DNA were 13.2%, 17.2% and 23.4% The positive rate of IgM was lower than that of MP-DNA in the same group (P <0.05), and the positive rate of MP-IgM was lower in> 3-6 years old group and> 6-14 years old group (P <0.05). Conclusion The immune system development of children of different ages is at different stages. There may be differences in the immune response to MP. Children should be selected appropriately according to the specific conditions and diagnosed as soon as possible.