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目的多重评价液氮冷冻加被动强化运动法建立股骨头坏死模型的效果。方法选用毕格犬8只,用液氮冷冻加术后被动强化运动法制备股骨头坏死模型(实验侧),对侧对照(对照侧)。术后1、4、8、16周取股骨头标本行苏木精-伊红染色;术后1、8、16周行MRI扫描;术前及处死前行数字减影血管造影术(DSA)。结果实验侧均出现不同程度股骨头坏死表现,且术后16周标本呈轻度塌陷;MRI示术后8周实验侧T2WI出现高信号,内部散在低信号影,术后16周低信号影进一步扩大;DSA示术后16周股骨头各支血管呈现不同的程度的狭窄或堵塞,并可见新生血管经股骨颈、髓腔等方向进入股骨头,但大多未能到达坏死区域。结论液氮冷冻加被动强化运动法建立的股骨头坏死模型符合股骨头坏死的病理变化,是一种比较理想的造模方法。
Objective To evaluate the effect of liquid nitrogen freezing and passive strengthening exercise to establish the model of femoral head necrosis. Eight Beagle dogs were selected. The model of femoral head necrosis was prepared by liquid nitrogen freezing plus passive exercise. The contralateral control (control side). At 1, 4, 8 and 16 weeks after operation, hematoxylin-eosin (HE) staining was performed on femoral heads and MRI scan was performed at 1, 8 and 16 weeks after operation. Digital subtraction angiography (DSA) . Results The experimental group showed varying degrees of osteonecrosis of the femoral head on the experimental side, and slightly collapsed at 16 weeks postoperatively. MRI showed high signal on T2WI and low signal on the 8th week after operation, DSA showed that the vessels of the femoral heads presented different degrees of stenosis or occlusion at 16 weeks postoperatively. The neovascularization showed that the neovascularization entered the femoral head through the femoral neck and medullary cavity, but most of them failed to reach the necrotic area. Conclusion The model of femoral head necrosis established by liquid nitrogen freezing plus passive strengthening exercise method is in line with the pathological changes of femoral head necrosis and is an ideal method of modeling.