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目的探讨未经药物治疗的初发系统性红斑狼疮患者Th1/Th2细胞亚群分布,白介素12、白介素18及其受体的基因表达。方法运用三色荧光标记流式细胞术检测35例初发SLE患者、10例正常人Th1/Th2细胞亚群分布;ABI7700Real-TimePCR法同时检测38例患者和28例正常人IL-12、IL-18及其受体mRNA表达水平。结果①初发狼疮患者Th1较正常人明显减低(P<0.05),但Th1/Th2无显著性改变。②与正常人组相比,SLE组患者IL-12、IL-18mRNA及其受体表达较正常人明显降低(P值均<0.05);③面部红斑组患者Th1/Th2、IL-12P35较正常人组降低(P均<0.05);④RNP阳性组患者IL-12P40较正常人组升高,IL-12P35、IL-18较正常人组降低(P均<0.05)。结论SLE是一种以Th1细胞下降,Th2细胞相对占优势的自身免疫性疾病,源于诱导向Th1细胞分化的一系列细胞因子及其受体减少和细胞因子间失衡所致。
Objective To investigate the distribution of Th1 / Th2 cell subsets, the expression of interleukin 12, interleukin 18 and their receptors in patients with newly diagnosed systemic lupus erythematosus without drug treatment. Methods The distribution of Th1 / Th2 subsets in 35 primary SLE patients and 10 normal controls was detected by using three-color fluorescence-labeled flow cytometry. The levels of IL-12 and IL-12 in 38 patients and 28 normal controls were detected by ABI7700 Real- 18 and its receptor mRNA expression levels. Results ① The Th1 level in newly diagnosed lupus patients was significantly lower than that in normal people (P <0.05), but there was no significant change in Th1 / Th2. ②Compared with normal control group, the expression of IL-12, IL-18 mRNA and its receptor in SLE group were significantly lower than those in normal control group (all P <0.05); ③ Th1 / Th2 and IL-12P35 in facial erythema group were more than normal (P <0.05). (4) The levels of IL-12P40 in RNP positive group were significantly higher than those in normal control group (P <0.05). IL-12P35 and IL- Conclusions SLE is an autoimmune disease with Th1 cells decline and Th2 cells predominate. It is derived from a series of cytokines and their receptors that induce differentiation into Th1 cells and the imbalance between cytokines.