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分别采用低浓度玉米小斑病菌Hel minthospolium maydis T小种毒素(HMT毒素)和玉米小斑病菌T小种培养滤液中的提取蛋白及菌丝细胞壁的提取液,预处理不同玉米品种3叶期叶片后再接种高浓度HMT毒素,在无菌条件下培养5 d后测量叶片病斑大小,并测定玉米叶片内苯丙氨酸解氨酶(PAL)活性变化。结果表明:只有纯化的低浓度HMT毒素能够作为激发子诱导玉米获得系统性广谱抗性,但不同玉米品种所需的最适合浓度有差异;TC103以质量浓度0.025μg/mL毒素预处理效果最好,PAL活性最高;TB37和TMo17 2个自交系,以质量浓度分别为0.025μg/mL和0.050μg/mL的毒素预处理效果最好。
The extracts from the filtrate of mycelium of Helminthosporium maydis T and the mycelial cell wall extracts of Helicoverpa zea were used to pretreat the leaf of 3-leaf stage of different maize cultivars After inoculation of high concentration of HMT toxin, the lesion size of leaves was measured after aseptic culture for 5 days, and the changes of phenylalanine ammonia lyase (PAL) activity in maize leaves were measured. The results showed that only purified low concentration of HMT toxin could induce systemic broad-spectrum resistance of maize as an elicitor, but the most suitable concentration required for different maize varieties was different. TC103 was the best pretreated with 0.025 μg / mL toxin The activity of PAL was the highest. The two inbred lines of TB37 and TMo17 had the best effect of toxin pretreatment at concentrations of 0.025μg / mL and 0.050μg / mL, respectively.