论文部分内容阅读
目的探讨宫颈鳞癌(SCC)局部肿瘤血管生成及其临床意义。方法应用免疫组化技术检测28例正常宫颈上皮(NCE),36例宫颈上皮内瘤变(CIN)和68例宫颈鳞癌组织局部微血管密度MVD(CD34标记)、细胞增殖指数PI(MIB-1标记)和微淋巴管密度LMVD(VEGFR-3标记)。结果 CD34标记的MVD在NCE、CIN及SCC组分别为(6.3±2.9)、(18.0±4.7)及(58.2±19.6)条。从NCE到CIN再到SCC,MVD显著升高(P<0.01)。在CIN中,MVD表达与PI显著正相关(r=0.373,P=0.025),而与LMVD无显著相关性(r=0.138,P=0.421)。在SCC中,MVD表达分别与PI及LMVD均无显著相关性(PI:r=-0.033,P=0.790;LMVD:r=0.114,P=0.354)。MVD在SCC组表达与间质浸润深度密切相关(P<0.01),而与患者年龄、FIGO分期、组织学分级、盆腔淋巴结转移、脉管浸润及生存率无关(P>0.05)。宫颈鳞癌突破深肌层间质浸润者MVD显著高于未突破深肌层间质浸润者(P<0.01)。结论 MVD表达上调可能促进宫颈异型上皮细胞增殖及宫颈鳞癌细胞浸润转移。
Objective To investigate the local tumor angiogenesis in cervical squamous cell carcinoma (SCC) and its clinical significance. Methods Immunohistochemical technique was used to detect the expression of MVD (MVD) in 28 cases of normal cervical epithelium (NCE), 36 cases of cervical intraepithelial neoplasia (CIN) and 68 cases of cervical squamous cell carcinoma, and the cell proliferative index (MIB-1 Marker) and lymphatic vessel density LMVD (VEGFR-3 marker). Results The CD34 - labeled MVD was (6.3 ± 2.9), (18.0 ± 4.7) and (58.2 ± 19.6) in the NCE, CIN and SCC groups, respectively. From NCE to CIN to SCC, MVD increased significantly (P <0.01). In CIN, MVD expression was positively correlated with PI (r = 0.373, P = 0.025), but not with LMVD (r = 0.138, P = 0.421). There was no significant correlation between MVD expression and PI and LMVD in SCC (PI: r = -0.033, P = 0.790; LMVD: r = 0.114, P = 0.354). The expression of MVD in SCC group was closely related to the depth of interstitial infiltration (P <0.01), but not to the age, FIGO stage, histological grade, pelvic lymph node metastasis, vascular invasion and survival rate (P> 0.05). The MVD of cervical squamous cell carcinoma that broke through the deep myometrial interstitial infiltration was significantly higher than those without invasion of the deep muscular layer (P <0.01). Conclusion The up-regulation of MVD may promote the proliferation of cervical atypical epithelial cells and the invasion and metastasis of cervical squamous cell carcinoma.