日本厚朴叶(5kg)甲醇提取物经氯仿和水萃取,氯仿层进一步经正己烷和90%甲醇萃取。甲醇提取部分上硅胶柱,用氯仿-甲醇(95∶9→2∶8)梯度洗脱,得到6个部分M-Ⅰ～M-Ⅵ。各部分上硅胶柱分离,共得到5个阿朴啡生物碱类化合物,分别为N-乙酰番荔枝碱(1)、N-乙酰木番荔枝碱(2)、N-甲酰番 Japanese Magnolia Leaf (5 kg) methanol extract was extracted with chloroform and water, and the chloroform layer was further extracted with n-hexane and 90% methanol. The methanol extracts were applied to a silica gel column and eluted with a gradient of chloroform-methanol (95: 9 → 2: 8) to give six fractions M-I to M-VI. All the parts were separated on silica gel column, and five aprin alkaloids were obtained, which were N-acetyl-lignin (1), N-acetyl- lignin (2)