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【目的】测定黄颡鱼源嗜水气单胞菌对盐酸沙拉沙星的耐药性,为制定盐酸沙拉沙星在水产动物疾病防治中的用药程序提供参考依据。【方法】采用二倍稀释法测定最小抑菌浓度(MIC),以传代法测定菌株的耐药性获得和消失速率。【结果】盐酸沙拉沙星对WZAh-01、WZAh-02、WZAh-03和YFAh-04菌株的MIC分别为0.625、0.3125、0.3125和0.15625μg/mL;在含有盐酸沙拉沙星的培养基中连续传代8次后,4株供试菌株的MIC上升了64～512倍;将获得的强耐药性诱变菌株于4℃中保存30 d,结果发现WZAh-02、WZAh-03和YFAh-04菌株的MIC下降了50%,WZAh-01菌株的MIC保持不变。【结论】黄颡鱼源嗜水气单胞菌经连续8代的耐药性诱导后对盐酸沙拉沙星获得很强的耐药性,且产生耐药突变后的菌株具有相当稳定的遗传性,耐药性在短期内很难消除,因此在实际生产上不宜常用或滥用盐酸沙拉沙星。
【Objective】 To determine the drug resistance of Aeromonas hydrophila to the sarafloxacin hydrochloride, and to provide a reference for the formulation of sarafloxacin hydrochloride in the control of aquatic animal diseases. 【Method】 The minimum inhibitory concentration (MIC) was determined by double dilution method. The drug resistance acquisition and disappearance rate was determined by the passaging method. 【Results】 The MICs of sarafloxacin hydrochloride to WZAh-01, WZAh-02, WZAh-03 and YFAh-04 were 0.625,0.3125,0.3125 and 0.15625μg / mL, respectively. The MICs of sarafloxacin hydrochloride in continuous medium containing sarafloxacin hydrochloride After passage 8 times, the MICs of the four tested strains increased by 64 to 512 times; the strong resistant mutants strains were stored at 4 ° C for 30 days and found that WZAh-02, WZAh-03 and YFAh-04 The MIC of the strain decreased by 50% and the MIC of WZAh-01 strain remained unchanged. 【Conclusion】 Aeromonas hydrophila strains were highly resistant to sarafloxacin hydrochloride after being induced by 8 generations of drug resistance, and the strains with the drug-resistant mutation had a fairly stable hereditary , Resistance in the short term is difficult to eliminate, so in actual production should not be used or abuse of sarafloxacin hydrochloride.