通过PCR和RACE技术克隆获得了巴西橡胶树染色质甲基化酶(CMT,chromomethylase)基因(Hb CMT1)。Hb CMT1全长2697 bp,含有2556 bp的阅读框,编码851个氨基酸。推测Hb CMT1分子量为95.67 k D,等电点为5.38,氨基酸序列与可可、烟草、葡萄、黄瓜、鹰嘴豆和拟南芥等CMT家族成员的同源性分别为66%、51%、50%、56%、53%和50%。定量PCR分析表明Hb CMT1在巴西橡胶树的根、树皮、叶、胶乳中均有表达,其中在叶中表达量最高,在胶乳中表达量最低。此外Hb CMT1在橡胶树自根幼态无性系胶乳中的表达量比老态无性系胶乳中的低。 Chromosomethylase (CMT) gene (Hb CMT1) was cloned by PCR and RACE techniques. Hb CMT1 is 2697 bp in length and contains a 2556 bp reading frame encoding 851 amino acids. It is speculated that the molecular weight of Hb CMT1 is 95.67 kD and the isoelectric point is 5.38. The amino acid sequence of Hb CMT1 is 66%, 51%, 50% of the CMT family member such as cocoa, tobacco, grape, cucumber, chickpea and Arabidopsis respectively %, 56%, 53% and 50%. Quantitative PCR analysis showed that Hb CMT1 was expressed in roots, barks, leaves and latexes of Hevea brasiliensis, with the highest expression in leaves and the lowest in latex. In addition, the expression level of Hb CMT1 in the rubbery root-primed clonal latex was lower than that in the clonal latex.